TCN1 Deficiency Inhibits the Malignancy of Colorectal Cancer Cells by Regulating the ITGB4 Pathway

Background/Aims@#This study aimed to investigate the biological function and regulatory mechanism of TCN1 in colorectal cancer (CRC). @*Methods@#We studied the biological function of TCN1 by performing gain-of-function and loss-offunction analyses in HCT116 cell lines; examined the effects of TCN1 on the proliferation, apoptosis, and invasion of CRC cells; and determined potential molecular mechanisms using HCT116 and SW480 CRC lines and mouse xenotransplantation models. Tumor xenograft and colonization assays were performed to detect the tumorigenicity and metastatic foci of cells in vivo. @*Results@#TCN1 knockdown attenuated CRC cell proliferation and invasion and promoted cell apoptosis. Overexpression of TCN1 yielded the opposite effects. In addition, TCN1-knockdown HCT116 cells failed to form metastatic foci in the peritoneum after intravenous injection. Molecular mechanism analyses showed that TCN1 interacted with integrin subunit β4 (ITGB4) to positively regulate the expression of ITGB4. TCN1 knockdown promoted the degradation of ITGB4 and increased the instability of ITGB4 and filamin A. Downregulation of ITGB4 at the protein level resulted in the disassociation of the ITGB4/plectin complex, leading to cytoskeletal damage. @*Conclusions@#TCN1 might play an oncogenic role in CRC by regulating the ITGB4 signaling pathway.

Analysis of anxiety and depressive status and influencing factors in patients with colorectal polyps in part of Xinjiang area / 中华消化杂志

Objective:To investigated the prevalence of anxiety and depression symptoms in patients with colorectal polyps in part of Xinjiang area and to explore the associated influencing factors related to psychological status, and to provide evidence and clues to promote the diagnosis and treatment of psychosomatic diseases in digestive system.Methods:From December 2021 to June 2022, at the First Affiliated Hospital of Shihezi University, the Fourth Division Hospital of Xinjiang Production and Construction Corps, the Third Division Hospital of Xinjiang Production and Construction Corps (Kashgar Hospital), the Thirteenth Division Red Star Hospital of Xinjiang Production and Construction Corps, the Tenth Division Beitun Hospital of Xinjiang Production and Construction Corps, the Seventh Division Hospital of Xinjiang Production and Construction Corps, the Fifth Division Hospital of Xinjiang Production and Construction Corps, patients who underwent colonoscopy and colorectal polyps detected were enrolled. The generalized anxiety disorder scale-7 (GAD-7) was used to screen anxiety symptoms, and the patient health questionaire-8 (PHQ-8) was performed to screen depression symptoms, and the general situation questionnaire was used to collect clinical information of patients. The questionnairs were completed via the Questionnaire Star platform or paper questionnaire, and a database was established. The patients were divided into groups according to whether with anxiety and depression symptoms. Multivariable logistic regression models was performed to analyze the factors related to anxiety and depression.Results:A total of 516 questionnaires were distributed and collected, among which 9 questionnaires were incomplete and 507 questionnaires were valid, the effective rate of questionnaires was 98.26%. The detection rates of anxiety and depression symptoms in 507 patients with colorectal polyps were 21.50%(109/507) and 19.33%(98/507), respectively. The results of multivariable logistic analysis revealed that female ( OR=3.87, 95% confidence interval (95% CI) 2.30 to 6.51, P<0.001), maximum diameter of polyp ( OR=1.74, 95% CI 1.14 to 2.67, P=0.011), perception of polyps as cancer ( OR=13.96, 95% CI 1.48 to 132.07, P=0.022), and the occurrence of gastrointestinal symptoms after polyp detection ( OR=5.43, 95% CI 1.74 to 16.92, P=0.004) were independent risk factors of anxiety in patients with colorectal polyps. Female ( OR=2.42, 95% CI 1.47 to 4.00, P=0.001), the number of polyps ( OR=1.07, 95% CI 1.01 to 1.13, P=0.028) and the count of gastrointestinal symptoms ( OR=2.04, 95% CI 1.34 to 3.10, P=0.001) were independent risk factors of depression in patients with colorectal polyps. Conclusion:Gender, polyp size, number of polyps, disease perception, and concomitant gastrointestinal symptoms are associated with anxiety and depression in patients with colorectal polyps.

Application research of PBL teaching based on POA theory in clinical practice teaching in the department of hand and foot surgery / 中华医学教育探索杂志

Objective:To explore the application value of problem-based learning (PBL) based on production-oriented approach (POA) theory in clinical practice teaching in department of hand and foot surgery.Methods:A total of 112 interns from the Department of Hand and Foot Surgery in Affiliated Hospital of Jining Medical University from January 2019 to October 2020 were randomly divided into control group and observation group, with 56 interns in each group. The control group was taught by PBL teaching, and the observation group was taught by PBL teaching based on POA theory. The two groups of interns were compared in terms of the evaluation of teaching effect, the statistics of errors in practice assessment, the assessment scale of independent learning ability, the career maturity inventory attitude scale, and the satisfaction of interns with their teachers. SPSS 25.0 was used for Chi-test and t-test. Results:The teaching effect and the satisfaction of interns with the teacher in the observation group were better than those of the control group ( P<0.05); the error rate of unskilled physical examination and the error rate of poor aseptic debridement concept in the observation group were all lower ( P<0.05); there was no significant difference in the error rate of irregular writing of medical records, the error rate of lack of medical history collection, and the error rate of communication errors between the two groups ( P>0.05). After teaching, the scores of independent learning ability and career maturity inventory attitude scale in the observation group were higher than those in the control group ( P<0.05). Conclusion:The PBL teaching based on POA theory has a good effect in the clinical practice teaching in the department of hand and foot surgery, and it is worthy of further promotion.

Changes analysis of CXCL12/CXCR4 in peripheral blood of patients with systemic lupus erythematosus before and after treatment / 中华风湿病学杂志

Objective:To observe the pattern change of chemokine (C-X-C motif) ligand 12 (CXCL12)/C-X-C chemokine receptor type 4 (CXCR4) in systemic lupus erythematosus (SLE) by comparing the condition of SLE patients between pre-treatment and post-treatment.Methods:Peripheral blood mononuclear cells were obtained from 15 healthy controls and 8 first visit SLE patients before and after treatment. The expression of CXCR4, CD3, and CD19 on lymphocytes were detected by flow cytometry. CXCL12 concentration in serum was measured by enzyme-linked immunosorbent assay. For statistical analysis, the independent sample t test was used for independent samples of normally distributed data, the paired sample t test was used for paired samples of normally distributed data, the Mann-Whitney U test was used for independent samples of nonnormal distribution data, and the Wilcoxon test was used for comparison of related samples of non-normal distribution data. In the correlation analysis, Pearson correlation coefficient was used for data conforming to the normal distribution, and Spearman correlation coefficient was used for data not conforming to the normal distribution. Results:① The percentage of CXCR4 + lymphocyte subsets was significantly increased after treatment (90±7)% when compared with that before treatment (75±17)% ( t=-2.440, P<0.05). ② The mean fluorescence intensity of CXCR4 + lymphocyte subsets was significantly decreased after treatment [119.00(92.93, 142.50)] compared with that before treatment [177.00(130.75, 280.25)] ( Z=-2.100, P<0.05). ③ The plasma concentration of CXCL12 was significantly decreased after treatment (540±243) pg/ml when compared with that before the treatment (1 146±570) pg/ml ( t=3.219, P<0.05). ④ Moreover, there were significant correlations between the trends of the above three indicators after treatment. Conclusion:The proportion, the mean fluorescence intensity of CXCR4 + lymphocyte in peripheal blood subsets and the concentration of CXCL12 in plasma of first visit SLE patients are significantly changed after treatment. That suggests CXCL12/CXCR4 is closely associated with SLE.

The sensation recovery of superficial palmar branch of the radial artery flap with palmar branch of median nerve and donor site / 中华显微外科杂志

Objective To explore the sen sation recovery of superficial palmar branc h of the radial artery flap with palmar branch of median nerve and donor si te. Methods From January, 2014 to June, 2016, 12 cases of finger soft tissue defects were repaired with sup erficial palmar branch of the radial artery flap with palmar branch of median nerve. The 2 g tactile sensation, 5 g pain sen sation, 30 g pressure sensation and static two-point discrimination (S2 -PD) of the flap was tested regularly. The S2 -PD of the palmar cutaneous branch of the median nerve were recorded respectively on the affected side and the unaffected side. The results were applied comparative t-test to perform statistical analysis, to observe the sensory recovery of the flap and donor site. Results The flaps survived.Twelve cases was followed-up for 6-18 mont hs. The pressure sensation of about 83.3％ of patients recovered after 2 months and 100％ of patients recovered after 3 months. The tactile sensation of about 91.7％ of patients recovered after 3 months and 100％ of patients recovered after 4 months. The pain sensation of about 91.7％ of patients recovered after 4 months of and 100％ of patients recovered after 5 months. S2-PD of the flap was the average of 8.3 mm in 6 months after operation. There were no significant differences in the S2-PD between the affected group [(12.08±2.15)mm] and unaffected group [(10.58±2.11)mm](P>0.05). And the sensory recovery of the control area of the palmar branch of the median nerve was S4 in 2 cases, S3+ in 9 cases, and S3 in 1 case. The sensory recovery was good. Conclusion Superficial palmar branch of the radial artery flap with palmar branch of median nerve can be used repair the skin defect of the fingers. The flap has a good sensery recovery, and the sensation of the donor area is gradually restored.

Effects of domestic wire-reinforced epidural catheter on complications related to anesthesia during labor analgesia / 中华麻醉学杂志

Objective To evaluate the effects of the domestic wire-reinforced epidural catheter on complications related to anesthesia during labor analgesia.Methods A total of 2 407 parturients,aged 18-40 yr,weighing 50-100 kg,of American Society of Anesthesiologists physical status Ⅰ or Ⅱ,scheduled for elective operations under combined spinal-epidural anesthesia for labor analgesia,were divided into 2 groups using a random number table:comnon epidural catheter group (group Ⅰ,n=1 203) and wirereinforced epidural catheter group (group Ⅱ,n=1 204).After successful epidural puncture,the appropriate amount of local anesthetics was injected into the subarachnoid space,and the corresponding epidural catheter was then inserted,and the depth was 3 em.Paresthesia during catheterization,unexpected catheter displacement,difficult extubation,catheter bending,epidural bleeding,successful insertion at first attempt,successful insertion time,the number of successfully delivered doses and consumption of patientcontrolled analgesia solution were recorded.The patients were followed up after operation for nerve damage.Results Compared with group Ⅰ,the incidence of paresthesia during catheterization,unexpected catheter displacement and catheter bending was significantly decreased,the occurrence of epidural bleeding was reduced,the success rate of insertion at first attempt was increased,successful insertion time was shortened,the incidence of difficult extubation was increased (P＜0.05 or 0.01),and no significant change was found in the number of successfully delivered doses,consumption of patient-controlled analgesia solution or incidence of nerve damage after operation in group Ⅱ (P＞0.05).Conclusion Complications related to anesthesia are reduced when the domestic wire-reinforced epidural catheter is used for labor analgesia.

Stem cells for the treatment of glaucoma / 眼科新进展

Glaucoma is a kind of disease with irreversible degeneration of optic nerve and visual field defects,and the mechanism of optic nerve injury caused by glaucoma has undefined at present;meanwhile,the treatment of glaucoma has not been achieving satisfying outcomes in clinical practices.It has been well known that stem cells have the ability to self-proliferate and differentiate.Recently,with the deepening of stem cell research,it is possible for humans to use stem cells to treat glaucoma.There are two methods of stem cell transplantation for glaucoma:stem cell replacement therapy for trabecular meshwork and retinal ganglion cell replacement therapy,so this article will give a review on the progress of stem cells in the treatment of glaucoma.

Inhibitory effect of Bletilla striata extracts on bleomycin-induced pulmonary fibrosis in rats / 国际药学研究杂志

Objective To investigate the inhibitory effect of Bletilla striata extracts(BSE)on bleomycin-induced pulmonary fibrosis(PF)in rats and study the chemical characteristics of the active component. Methods B. striata was extracted with 95%ethyl?alcohol to obtain the ethanol extract. The residue was extracted with water and precipitated with ethanol to obtain the B. striata polysac?charide(BSP). Sprague Dawley male rats were intratracheally administered of bleomycin(BLM,5 mg/kg)to induce PF. PF Rats were administered with ethanol BSE(300 mg/kg body mass)and BSP(300 mg/kg body mass)once daily for 28 d by gavage. On 14 d and 28 d, the lungs were harvested to observe the pathological and collagen deposition changes. The lung coefficient and the content of hydroxy?proline(HYP)were also determined. The phenol-sulfuric acid method was used to detect the total sugar content,the relative molecular mass was detected by high performance gel permeation chromatography(HPGPC)and the monosaccharide components were analyzed by high performance liquid chromatography(HPLC). Results Compared with PF model group,the histopathological changes in lung tissue treated with BSP group were obviously improved ,the level of HYP in lung tissues and the lung coefficient were significantly decreased (P<0.05,P<0.01). But the group treated with ethanol BSE had no significant improvement. The content of BSP was 81.41%,and the number average molecular weight(Mn)of BSP was about 2.23 × 105. The BSP was mainly composed of glucose and mannose. Conclusion HYP could significantly decrease lung HYB and collagen deposition of PF rats,and has the potiential for inhi?bition of PF.

Effect of Euryale ferox seeds ethanol extract on renal function of diabetic nephropathy rats and determination of its antioxidant ability in vitro / 国际药学研究杂志

Objective To investigate the effect of Euryale ferox seeds ethanol extract on renal funtion of diabetic nephropathy (DN) induced by strepotozotocin (STZ) in rats and the antioxidant ability of ethanol extract,water extract supernatant and precipitation. Methods Ethanol extract of Euryale ferox seeds were prepared by ethanol extraction and followed by evaporation and freeze-dry. The seeds residue was further extracted with water and precipitated with ethanol to obtain water extract supernatant and precipitation. Sprague Dawley male rats were given intraperitoneal injection of STZ at a dose of 60 mg/kg to induce DN. STZ-induced DN rats were administrated by gavage with different dose of Euryale ferox seeds ethanol extract(75, 150, 300 and 600 mg/kg body weight) once daily for 12 weeks. 24 h urine was collected for examining urinary protein and microalbumin every four weeks. Blood serum was also collected for examining of serum creatinine (Cr) and blood urea nitrogen (BUN) level after rats sacrificed at the end of 12 weeks after administration, and the pathological changes of kidney were observed. Antioxidant capacity of the three extracts were determined by Fe3+ reduction, and radical scavenging rate to 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazl(DPPH), O2-and OH-free radicals. Results Compared with model group, the content of 24 h urine protein and urine microalbumin of DN rats were reduced in all Euryale ferox seeds ethanol extract administration groups, and 24 h proteinuria were significantly reduced in the group of 150 and 600 mg/kg administration(P<0.05);BUN level was also reduced in all dose of Euayale ferox seeds ethanol extract administration, and more remarkably reduced in the group of 300mg/kg (P<0.01). 12 weeks after administration, kidney damage amelioration was observed in pathological sections of rats in Euryale ferox seeds ethanol extracts 300 and 600 mg/kg groups. Meanwhile, Euryale ferox seeds ethanol extract showed a certain antioxidant abilities in reducing of Fe3+ and scavenging DPPH, O2- and OH- at the concentrations of 0.6, 0.8 and 1.0 mg/ml. Conclusion Euryale ferox seeds ethanol extract could decrease proteinuria of STZ-induced DN rats,this may be associated with its antioxidant capacity.

Vitamin D and the recurrence of rheumatoid arthritis / 中国医师进修杂志

Objective To identify the correlation between vitamin D prescription and recurrence in rheumatoid arthritis (RA).Methods A randomized controlled trial study was performed in 170 RA patients who were in remission during the past 2 months.According to the level of vitamin D,170 RA patients were divided into normal vitamin D group (84 patients) and deficiency vitamin D group (86 patients).Deficiency vitamin D group were randomly allocated to receive vitamin D treatment(vitamin D treatment group) or without vitamin D treatment (control group).In the 6-month follow-up period,the recurrence status was observed and compared.Results In the 6-month follow-up period,the recurrence rate of RA in normal vitamin D group was 16.7％(14/84),in vitamin D treatment group was 19.0％(8/42) and in control group was 29.5％(13/44),and there was no significant difference (P ＞ 0.05).In vitamin D treatment group,no hypercalcemia and hyperphosphatemia occured.The age,course of disease and remission time in recurrence patients of three groups were no significant difference (P ＞ 0.05).The level of vitamin D in recurrence patients of vitamin D treatment group was higher than that in recurrence patients of control group:(25.5 ± 8.9) ng/L vs.(20.9 ± 8.6)ng/L,and there was significant difference(P＜ 0.05).Conclusion Vitamin D deficiency is not identified to be a risk factor for RA recurrence.Vitamin D does not reduce the recurrence of RA.

Reconstruction of bone and skin defect at medial malleolns / 中华显微外科杂志

Objective To investigate the operative method and clinical rusults of repairing and reconstruction for bone and skin defect at medial malleolus.Methods Form January,2013 to January,2015,11 cases of patients with malleolus complex tissue defect were treated.According to the degree of damage to the cases were divided into four types,according to the type,selected flap,blood vessels,nerves,tendon graft,flap series connection iliac bone flap graft to repair,or direct ankle fusion,amputation to therapy.The Iliac flap donor site remained part of the anterior superior iliac spine and rebuild muscle starting and ending point,the donor sites wound was closed by skin graft.Results Followed-up of 1-24 months,in addition to amputation and ankle fusion each one exception,the remaining 9 patients underwent ankle reconstruction to reserve ankle.One case acquired infection,other 8 patients healed well,had different degree of recovery of the ankle function,the ankle function had recovery in different degree.AOFAS ankle-hindfoot score system:excellent in 4 cases,good in 4 cases,and poor in 1 case.Conclusion Ankle complex tissue defect classification method can be used to guide us to select the treatment options.Iliac bone flap series consisting mainly of composite tissue transplantation is a viable method of the medial malleolus defect reconstruction.

Oxidative stress effect of HPM on brain of rats / 军事医学

Objective To investigate the adverse effect of different doses of high power microwave(HPM) irradiation on oxidative stress in the brain of Wistar rats in order to contribute to establishing an animal model to evaluate protective agents which will be used for protection against microwave radiation.Methods Eighty male Wistar rats were randomly divided into 16 groups according to factor analysis.The average power density was 0,10,30 and 100 mW/cm2 and the sampling time was 6 h,1,3 and 7 d .The duration of exposure was 6 minutes for each radiation group.After exposure, the rats were sacrificed at each sampling time.Colorimetric method was used to measure the content of malondialdehyde(MDA) and protein carbonyl, the activity of GSH-px, SOD and CAT.Results The content of MDA and protein carbonyl of each radiation group was increased with the radiation dose, but decreased with the sampling time prolonged.The activity of superoxide dismutast(SOD),glutathion peroxidase(GSH-px) and catalase(CAT) in each radiation group was decreased with the radiation dose increased, and with the sampling time prolonged, but increased later.Conclusion Microwave radiation can cause oxidative stress in rats brain, as shown by the oxidative damage of lipid and protein and the decrease in the activity of antioxidant enzymes.Besides, the effect also depends on the radiation dose and sampling time.

Research progress in Euryale ferox:a Chinese herb / 国际药学研究杂志

The seed, coat, petiole and peduncle of Euryale ferox(belonging to Nymphaeaceae) are used as medicinal potions through solvent extraction, reflux extraction and ultrasonic extraction, and purification on macroporous resin. The quality controls are performed by near infra-red fingerprint spectrum, HPLC, X-ray diffraction Fourier spectrum, and ITS2 barcode. The nutrients of E. ferox contain large amount of amino acids, fatty acids and various microelements; medicinal ingredients are identified to be polyphenol, sesquineolignan, tocopherol, cerebroside, and cyclic dipeptide. The pharmacological effects of E. ferox extracts include antioxidant, free radical scavenging, hypoglycemic activity, decreasing urine protein, bacteriostasis and prevention of gastric mucosal injury. Treatment with E. ferox preparations for diabetes and chronic nephritis are performed in clinics. This review summarizes the study of E. ferox in recent years for further research and development.

Expression and clinical significance of cysteine-X-cysteine chemokine receptor 4, and stromal cell derived factor 1α in the peripheral blood of patients with systemic lupus erythematosus / 中华风湿病学杂志

Objective To explore the expressions of stromal cell derived factor 1 α (SDF-1α) and cysteine-X-cysteine chemokine receptor 4 (CXCR4) in the peripheral blood of patients with systemic lupus erythematosus (SLE).Methods Forty hospitalized SLE patients were recruited and twenty healthy volunteers were enrolled as normal controls.The percentage of CD3+CD4+CXCR4+,CD3+CD8+CXCR4+ and the plasma concentration level of SDF-1α in the control group and SLE patients were detected by flow cytometry and ELISA.The relationship between SDF-1α/CXCR4 and SLEDAI was explored.Kruskal-Wallis H,Pearson's and Mann-Whitney U test were used for statistical analysis.Results the expression of SDF-1α [329 (127,539) pg/ml] and CXCR4 [CD3+CD4+CXCR4+:4.20(2.01,6.35)％,CD3+CD4+CXCR4+:2.70(1.68,4.20)％] were significantly elevated in SLE patients when compared to the normal controls (Z=-6.277,-5.707,-4.885,respectively,all P=0.000),and were significantly increased in highly active SLE patients than less active SLE (Z=-5.414,-5.256,-5.312,P＜0.01).The expression of SDF-1α and CXCR4 in the butterfly erythema group,anemia group and proteinuria group were significantly higher than the normal group (P＜0.05).Both SDF-1αand CXCR4 were positively correlated with SLEDAI (r=0.857,0.830,0.861,respectively,all P＜0.01).Conclusion The expressions of SDF-1α and CXCR4 increase significantly in the peripheral blood of SLE patients and there is close relationship between SDF-1α/CXCR4 and disease activity,organ damage.The results of this study suggestthat SDF-1α/CXCR4 may play an important role in the pathogenesis of SLE.

The establishment of canine acute obstructive hydrocephalus model and experimental study of the best treatment / 中国综合临床

Objective To gain more pathophysiolgic knowledge about acute obstructive hydrocephalus and to explore its rapid and effective treatment by establishing canine acute obstructive hydrocephalus model.Methods Acute obstructive hydrocephalus model was established by injecting cyan-acrylic gel glue into the fourth ventricle via posterior fosse craniotomy in 9 male adult mongrel dogs.At the same time,lateral ventricle catheterization were performed and were fixed on the scalp to connect reservoir bag so that the changes of intracranial pressure (ICP) could be measured dynamically,and the changes of neurological function were observed.Results Acute obstructive hydrocephalus model was successfully established in 6 of the total 9dogs.ICP was (48.2 ± 6.1 ) cm H2 O at 48 hours after the injection and was (56.4 ± 5.7 ) cm H2 O at 72 hours after the injection,it increased 392％ and 459 ％ respectively.And the ICP after injection was significantly different(P ＜ 0.01 )compared with that before injection (12.3 ± 3.1 )cm H2O.Conclusion The establishment of acute obstructive hydrocephalus model has high success rate,and is easy to reduplicate; ICP could be measured dynamically and also could be reduced by releasing CSF;Thus,ventricular drainage is the most rapid and effective treatment for acute obstructive hydrocephalus.

Pilot-scale production of recombinant plasmid pUDK-HGF / 生物工程学报

pUDK-HGF, the recombinant plasmid DNA encoding human hepatocyte growth factor (HGF), can treat ischaemic disease. A great quantity of pharmaceutical pUDK-HGF is needed. A pilot-scale production process of pUDK-HGF was established based on a new chromatographic media (plasmidselect), including fermentation, cell harvesting, alkaline lysis, ultrafiltration, RNA removing and buffer exchanging on Sephacryl S-1000, capturing supercoiled plasmid DNA with plasmidselect, and removing the salt with Sepharose 6BFF. The process does not use RNase enzyme and toxic solvents.

Establishment of multidrug-resistance cell line C(6)/adr and reversal of drug-resistance / 中华医学杂志(英文版)

<p><b>OBJECTIVE</b>To investigate the mechanism of multidrug resistance (MDR) in a human glioma cell and methods for overcoming multi-drug resistance.</p><p><b>METHODS</b>MDR cell line C(6)/adr was established. The expression of the mdr-1 gene and its P-glycoprotein (P-gp) in the C(6)/adr cell line was observed by RT-PCR and flow cytometry. The reversal of MDR by verapamil, erythromycin, dihydropyridine, P-gp monoclonal antibody and Salvia miltiorrhiza (SM) was studied by microtiter tetrazolium (MTT) assay or by high performance liquid chromatographic assay.</p><p><b>RESULTS</b>The mdr-1 gene of the C(6)/adr cell line was positive, over-expressing P-gp. The drug-resistance of the C(6)/adr cell lines could be partly reversed by 2 - 6 microg/ml of verapamil, 50 - 100 microg/ml of erythromycin, or 5 microg/ml of dihydropyridine. As concentration increased, they had a better effect. Among these drugs, 100 microg/ml of erythromycin had the best result of reversal. Dihydropyridine 1 microg/ml, P-gp monoclonal antibody and SM had no effect.</p><p><b>CONCLUSION</b>The mdr-1 gene and its expression might be associated with the MDR of glioma cells. Verapamil, erythromycin and dihydropyridine could reverse the MDR of glioma cells.</p>

The role of interleukin 6 and interleukin 6 receptor in gliomas / 中国癌症杂志

Purpose:To discuss the role of interleukin 6 (IL 6) and IL 6 receptor (IL 6R) in gliomas. Methods:We detected IL 6 and IL 6R gene in 30 human glioma tissue specimens and adult normal astrocytes. A complementary DNA copy of total RNA was synthesized and amplified with specific primers using the reverse transcription polymerase chain reaction (RT PCR) method. Results:IL 6 gene was positive of in 24 (80.0%) samples of gliomas, while IL 6R gene was positive of in 26 (86.7%) cases. Co expression of IL 6 and IL 6R was identified in 22 (73.3%) specimens of gliomas. On the other hand,the expression of IL 6 was weakly positive in adult normal astrocyte, but IL 6R was negative.Conclusions:Our results indicate that IL 6/IL 6R autocrine or paracrine loop may exist in glioma, which could enhance the glioma cell proliferation.

The effect of pericardial devascularization with vagustrunk preservation on portal hypertension gastropathy / 中国现代普通外科进展

Objective:To probe the different influence of pericardial devascularization by preserving vagus trunk(VTPPD) and pericardial devascularization (PD) on portal hypertensive gastropathy (PHG).Methods:77 patients with portal hypertension were divided into VTPPD and PD group,the VTPPD group included 36 cases,and PD group included 41 cases.Varices of esophagus and fundus of stomach and PHG were observed by gastroscopy before and 3 weeks after operation in all cases,and compared postoperative incidence of PHG in the 2 groups.Results:In all cases,Varices of esophagus and fundus of stomach disappeared or relieved obviously.The incidence of PHG in VTPPD group before operation was 55.6%(20/36),and that after operation was 69.4%(25/36),the former was not higher statistically(P=0.224);the incidence of PHG in PD group before operation was 61.0%(25/41),and that after operation was 87.8%(36/41),the former was not higher than the latter statistically(P=0.005);and the postoperative incidence of PHG in PD group was higher significantly than that in VTPPD group (P=0.048).There were 8(22.2%,8/36)patients whose degree of PHG aggravated in VTPPD group,and there were 19(46.3%,19/41)patients whose degree of PHG aggravated in PD group,the rate of the former was significantly lower than that of the latter(P=0.027).Conclusion:Comparing with the classic portoazygous devascularization,VTPPD can reduce the incidence and the degree of PHG.

Clinical Study on Recurrent Intracranial Aneurysm with Endovascular Embolization Treatment / 实用放射学杂志

Objective To evaluate the clinical factors of recurrent intracranial aneurysm with endovascular embolization.Methods DSA was performed in 70 cases with 74 intracranial aneurysms after endovascular embolized treatment,of them,8 cases(9 aneurysms)were detected having recurrent.The geometry form of aneurysms,embilized method,material of endovascular embolization and DSA images pre-and post-embolized were analysed.Results In all recurrent intracranial aneurysms,five located at posterior communicating artery,two at anterior communicating artery and two at the first crotch of right middle cerebral artery.Four were wide-necked aneurysms,five were irregular aneurysms in form.Before recurrence,three aneurysms were embolized completely,six were proportion occlusion.Conclusion Aneurysms localized at the branching region of artery,being wide-neck,large,irregular form and residual neck are facility factors for intracranial aneurysm recurrence after endovascular embolization.